Journal: ACS chemical neuroscience

Article Title: Small molecules targeting PTPσ—Trk interactions promote sympathetic nerve regeneration

doi: 10.1021/acschemneuro.1c00854

Figure Lengend Snippet: Representative western blots (left) probing for PTPσ and TrkA following TrkA-RFP immunoprecipitation. HEK-293t cells were transfected with TrkA-RFP and PTPσ, treated with either vehicle (DMSO), (A) HJ-01, (B) HJ-02, or (C) HJ-03 then stimulated with CSPGs and NGF. Quantification (right) of PTPσ that co-immunoprecipitated with TrkA-RFP normalized to vehicle treated cells. HJ-01 and HJ-02 reduced the amount of PTPσ that bound to TrkA-RFP at 1μM, but HJ-03 had no effect. (D) HEK-293t cells were transfected with TrkA-RFP and p75NTR, treated with vehicle (DMSO) and HJ-02, then stimulated with CSPGs and NGF. Quantification (right) of p75NTR that co-immunoprecipitated with TrkA-RFP normalized to vehicle treated cells. Data are mean ± SD, n=5-9 experiments; n.s.- not significant, *p<.05, **p<.01 vs. vehicle treated control, one-way ANOVA (Dunnett multiple comparisons post-test).

Article Snippet: We would like to thank Dr. Moses Chao (Skirball Institute, NYU) for the HEK-TrkB cells and for the TrkA-RFP and p75NTR plasmids (Addgene plasmid # 24093, 24091).

Techniques: Western Blot, Immunoprecipitation, Transfection

Journal: Cell Stem Cell

Article Title: Selective advantage of mutant stem cells in human clonal hematopoiesis is associated with attenuated response to inflammation and aging

doi: 10.1016/j.stem.2024.05.010

Figure Lengend Snippet:

Article Snippet: APC anti-human CD271 (clone ME20.4-1.H4) , Miltenyi , Cat# 130-113-418; RRID: AB_2733363.

Techniques: Recombinant, Reverse Transcription, DNA Library Preparation, DNA Sequencing, RNA Sequencing, Purification, Sequencing, Software, Sterility

Journal: Molecular medicine reports

Article Title: β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkA NGFR.

doi: 10.3892/mmr.2013.1590

Figure Lengend Snippet: Figure 1. (A) NGF, (B) TrkANGFR and (C) p75NTR protein expression in L-02 cells (immunocytochemistry; magnification, x200). NGF, nerve growth factor; TrkANGFR, tyrosine kinase-A nerve growth factor receptor; p75NTR, p75 pan-neurotrophin receptor.

Article Snippet: Recombinant human β-NGF was obtained from R&D Systems (Minneapolis, MN, USA) and rabbit polyclonal anti-NGF, anti-TrkANGFR and anti-p75NTR antibodies were derived from Boster Bioengineering Limited Company (Wuhan, China).

Techniques: Expressing, Immunocytochemistry

Journal: Molecular medicine reports

Article Title: β-Nerve growth factor attenuates hepatocyte injury induced by D-galactosamine in vitro via TrkA NGFR.

doi: 10.3892/mmr.2013.1590

Figure Lengend Snippet: Figure 3. β-NGF promotes the proliferation of L-02 cells. (A) The effect of β-NGF on the proliferation of L-02 cells injured by 40 mmol/l of D-GalN after 24 h (*P<0.05 vs. D-GalN). (B) The effect of β-NGF promoting the proliferation of L-02 cells was eradicated by an anti-TrkANGFR blocking antibody (*P<0.01 vs. β-NGF + GalN). Con, control; β-NGF, β-nerve growth factor; D-GalN, D-galactosamine; anti-TrkANGFR, anti-tyrosine kinase A nerve growth factor receptor; p75NTR, p75 pan-neurotrophin receptor; sily, silymarin. Values are presented as the mean ± SD (n=4).

Article Snippet: Recombinant human β-NGF was obtained from R&D Systems (Minneapolis, MN, USA) and rabbit polyclonal anti-NGF, anti-TrkANGFR and anti-p75NTR antibodies were derived from Boster Bioengineering Limited Company (Wuhan, China).

Techniques: Blocking Assay, Control

Journal: Cancers

Article Title: Adapter Chimeric Antigen Receptor (AdCAR)-Engineered NK-92 Cells for the Multiplex Targeting of Bone Metastases

doi: 10.3390/cancers13051124

Figure Lengend Snippet: Cell line immunophentoyping. Tumor cells were co-incubated with primary biotinylated antibodies. Antigen expression was detected using a secondary PE-coupled anti-biotin antibody. Percentage of stained cells and median fluorescence index (MFI) values were calculated using staining with the secondary antibody alone as negative control.

Article Snippet: Antigen Clone Antibody Order # Lot Supplier CD146 541-10B2 n/a 130-092-852 5190627154 Miltenyi Biotec, Bergisch Gladbach, Germany CD171 REA163 n/a 130-100-702 5190607129 Miltenyi Biotec, Bergisch Gladbach, Germany CD200 OX-104 n/a 130-106-064 5191021606 Miltenyi Biotec, Bergisch Gladbach, Germany CD221 REA271 n/a 130-103-973 5190627184 Miltenyi Biotec, Bergisch Gladbach, Germany CD271 REA844 n/a 130-112-608 5190627191 Miltenyi Biotec, Bergisch Gladbach, Germany CD274 n/a Atezolizumab n/a n/a Hoffmann-La Roche, Basel, Switzerland CD276 FM276 n/a 130-095-514 5190627174 Miltenyi Biotec, Bergisch Gladbach, Germany CD340 n/a Trastuzumab n/a n/a Hoffmann-La Roche, Basel, Switzerland EGFR n/a Cetuximab n/a n/a Merck KgaA, Darmstadt, Germany GD2 n/a Dinutuximab beta n/a n/a Eusa Pharma, Hertfordshire, Great Britain

Techniques: Expressing, Staining, Fluorescence, Control